求胡夏歌曲百度网盘:酶切位点保护碱基

酶

寡核苷酸序列

切割率%

2 hr

20 hr

Not I

TTGCGGCCGCAA
ATTTGCGGCCGCTTTA
AAATATGCGGCCGCTATAAA
ATAAGAATGCGGCCGCTAAACTAT
AAGGAAAAAAGCGGCCGCAAAAGGAAAA

0
10
10
25
25

0
10
10
90
>90

Nsi I

TGCATGCATGCA
CCAATGCATTGGTTCTGCAGTT

10
>90

>90
>90

Pac I

TTAATTAA
GTTAATTAAC
CCTTAATTAAGG

0
0
0

0
25
>90

Pme I

GTTTAAAC
GGTTTAAACC
GGGTTTAAACCC
AGCTTTGTTTAAACGGCGCGCCGG

0
0
0
75

0
25
50
>90

Pst I

GCTGCAGC
TGCACTGCAGTGCA
AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT

0
10
>90
>90
0

0
10
>90
>90
0

Pvu I

CCGATCGG
ATCGATCGAT
TCGCGATCGCGA

0
10
0

0
25
10

Sac I

CGAGCTCG

10

10

Sac II

GCCGCGGC
TCCCCGCGGGGA

0
50

0
>90

Sal I

GTCGACGTCAAAAGGCCATAGCGGCCGC
GCGTCGACGTCTTGGCCATAGCGGCCGCGG
ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA

0
10
10

0
50
75

Sca I

GAGTACTC
AAAAGTACTTTT

10
75

25
75

Sma I

CCCGGG
CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA

0
0
10
>90

10
10
50
>90

Spe I

GACTAGTC
GGACTAGTCC
CGGACTAGTCCG
CTAGACTAGTCTAG

10
10
0
0

>90
>90
50
50

Sph I

GGCATGCC
CATGCATGCATG
ACATGCATGCATGT

0
0
10

0
25
50

Stu I

AAGGCCTT
GAAGGCCTTC
AAAAGGCCTTTT

>90
>90
>90

>90
>90
>90

Xba I

CTCTAGAG
GCTCTAGAGC
TGCTCTAGAGCA
CTAGTCTAGACTAG

0
>90
75
75

0
>90
>90
>90

Xho I

CCTCGAGG
CCCTCGAGGG
CCGCTCGAGCGG

0
10
10

0
25
75

Xma I

CCCCGGGG
CCCCCGGGGG
CCCCCCGGGGGG
TCCCCCCGGGGGGA

0
25
50
>90

0
75
>90
>90

酶

寡核苷酸序列

切割率%

2 hr

20 hr

Acc I

GGTCGACC
CGGTCGACCG
CCGGTCGACCGG

0
0
0

0
0
0

Afl III

CACATGTG
CCACATGTGG
CCCACATGTGGG

0
>90
>90

0
>90
>90

Asc I

GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA

>90
>90
>90

>90
>90
>90

Ava I

CCCCGGGG
CCCCCGGGGG
TCCCCCGGGGGA

50
>90
>90

>90
>90
>90

BamH I

CGGATCCG
CGGGATCCCG
CGCGGATCCGCG

10
>90
>90

25
>90
>90

Bgl II

CAGATCTG
GAAGATCTTC
GGAAGATCTTCC

0
75
25

0
>90
>90

BssH II

GGCGCGCC
AGGCGCGCCT
TTGGCGCGCCAA

0
0
50

0
0
>90

BstE II

GGGT(A/T)ACCC

0

10

BstX I

AACTGCAGAACCAATGCATTGG
AAAACTGCAGCCAATGCATTGGAA
CTGCAGAACCAATGCATTGGATGCAT

0
25
25

0
50
>90

Cla I

CATCGATG
GATCGATC
CCATCGATGG
CCCATCGATGGG

0
0
>90
50

0
0
>90
50

EcoR I

GGAATTCC
CGGAATTCCG
CCGGAATTCCGG

>90
>90
>90

>90
>90
>90

Hae III

GGGGCCCC
AGCGGCCGCT
TTGCGGCCGCAA

>90
>90
>90

>90
>90
>90

Hind III

CAAGCTTG
CCAAGCTTGG
CCCAAGCTTGGG

0
0
10

0
0
75

Kpn I

GGGTACCC
GGGGTACCCC
CGGGGTACCCCG

0
>90
>90

0
>90
>90

Mlu I

GACGCGTC
CGACGCGTCG

0
25

0
50

Nco I

CCCATGGG
CATGCCATGGCATG

0
50

0
75

Nde I

CCATATGG
CCCATATGGG
CGCCATATGGCG
GGGTTTCATATGAAACCC
GGAATTCCATATGGAATTCC
GGGAATTCCATATGGAATTCCC

0
0
0
0
75
75

0
0
0
0
>90
>90

Nhe I

GGCTAGCC
CGGCTAGCCG
CTAGCTAGCTAG

0
10
10

0
25
50